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cd2 antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Miltenyi Biotec cd2 antibody, anti-human, reafinity
    Cd2 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd2/CD2+Antibody%2C+anti-human%2C+REAfinity/custom%40130-119-507%4042502392
    Average 93 stars, based on 4 article reviews
    cd2 antibody, anti-human, reafinity - by Bioz Stars, 2026-10
    93/100 stars

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    Selection:

    Article Title: Off-the-shelf dual CAR-iNKT cell immunotherapy eradicates medullary and leptomeningeal high-risk KMT2A -rearranged leukemia
    Article Snippet: .. CAR-iNKT cells were enriched by magnetic bead selection following 246 the manufacturers protocol against human CD2 (Miltenyi 130-091-114). .. CD2 positive cells 247 were then further purified by FACS sorting staining using Labelling Check Reagent (Miltenyi 248 8 130-122-219) and 7AAD viability stain (Cayman 11397) with additional antibodies against 249 mouse-CD45, human-CD45, and human CD19.

    Article Title: Bi-specific CAR-iNKT cell immunotherapy for high-risk KMT2A-rearranged leukemia outperforms CAR-T in an NKG2D-dependent manner and eradicates leptomeningeal disease
    Article Snippet: .. CAR-iNKT cells were enriched by magnetic bead selection following the manufacturers protocol against human CD2 (Miltenyi 130-091-114). .. CD2 positive cells were then further purified by FACS sorting staining using Labelling Check Reagent (Miltenyi 130-122-219) and 7AAD viability stain (Cayman 11397) with additional antibodies against mouse-CD45, human-CD45, and human CD19.

    Incubation:

    Article Title: Optimization of Human Mesenchymal Stem Cells for Rheumatoid Arthritis: Implications for Improved Therapeutic Outcomes.
    Article Snippet: .. Cells were stimulated using biotinylated antibodies directed against human CD2, CD3, and CD28 according to the manufacturer’s instructions (Miltenyi Biotec), with the range of CM, and incubated at 37 C in 5%CO2 for 4 days. .. CD4+ T cells were loaded with Cell Proliferation Dye eFluor 670 according to the manufacturer’s instructions (eBioscience), and proliferation rates were measured by flow cytometer BD LSR II (BD Biosciences) or Attune NxT (Thermo Fisher Scientific).

    Article Title: Optimization of Human Mesenchymal Stem Cells for Rheumatoid Arthritis: Implications for Improved Therapeutic Outcomes
    Article Snippet: .. Cells were stimulated using biotinylated antibodies directed against human CD2, CD3, and CD28 according to the manufacturer's instructions (Miltenyi Biotec), with the range of CM, and incubated at 37°C in 5% CO 2 for 4 days. .. CD4+ T cells were loaded with Cell Proliferation Dye eFluor 670 according to the manufacturer's instructions (eBioscience), and proliferation rates were measured by flow cytometer BD LSR II (BD Biosciences) or Attune NxT (Thermo Fisher Scientific).

    Article Title: Directs effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on adaptive immunogenesis
    Article Snippet: Cells were counted using a Z2 Cell and Particle counter (Beckman Coulter Inc., Fullerton, USA). .. Cell cultures Isolated CD14-positive cells were cultured at 1.0–1.5 × 10 6 /ml in 24-well plates in the presence or absence of bacterial LPS (1.0 μg/ml, Salmonella typhi , Pyrogenalum, Medgamal, Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, Russian Academy of Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 5.0 × 10 −5 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a humidified CO 2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0–1.5 × 10 6 /ml in the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against human CD2, CD3, and CD28 (T Cell Activation/Expansion Kit, human, MACS Miltenyi Biotec) in the same cell culture medium for 48 h. Both Mc/Mph and T cell cultures were incubated in the presence (test cultures) or absence (negative controls) of a tenfold dilution series of recombinant human GM-CSF (0.01; 0.1; 1.0, and 10.0 ng/ml). .. Flow cytometry Surface characteristics of Mc/Mph cells were studied by staining with a cocktail of the following specific monoclonal antibody ( Ab )-based reagents: peridinin chlorophyll (PerCP)-labeled anti-CD14 (eBioscience, USA), fluorescein isothiocyanate (FITC)-labeled anti-CD16, phycoerythrin (PE)-labeled anti-CD119, allophycocyanin (APC)-labeled anti-CD124, and PE/Alexa Fluor® 488-labeled anti-CD197 (BioLegend, San Diego, CA).

    Proliferation Assay:

    Article Title: Cutting Edge: Recruitment, Retention, and Migration Underpin Functional Phenotypic Heterogeneity of Regulatory T Cells in Tumors.
    Article Snippet: Before cell transfer and at 8 and 16 h afterward, ear thickness was measured with a thickness gauge (Teclock). .. Effector T cell proliferation assay CD41 effector and CD4 feeder cells were isolated from human CD2 spleen cells using human CD2 and CD4 MicroBeads (Miltenyi Biotec). .. After the CellTrace Violet (Thermo Fisher Scientific) staining of the CD41 cells, 625 or 1250 spleen Treg cells, KikGR-Red , or KikGR-Red1 tumor Tregs were cocultured with 5000 CD41 and 20- Gy irradiated 50,000 CD4 cells for 66 h in the presence of 500 ng/ ml anti-CD3 Ab (BioLegend).

    Isolation:

    Article Title: Cutting Edge: Recruitment, Retention, and Migration Underpin Functional Phenotypic Heterogeneity of Regulatory T Cells in Tumors.
    Article Snippet: Before cell transfer and at 8 and 16 h afterward, ear thickness was measured with a thickness gauge (Teclock). .. Effector T cell proliferation assay CD41 effector and CD4 feeder cells were isolated from human CD2 spleen cells using human CD2 and CD4 MicroBeads (Miltenyi Biotec). .. After the CellTrace Violet (Thermo Fisher Scientific) staining of the CD41 cells, 625 or 1250 spleen Treg cells, KikGR-Red , or KikGR-Red1 tumor Tregs were cocultured with 5000 CD41 and 20- Gy irradiated 50,000 CD4 cells for 66 h in the presence of 500 ng/ ml anti-CD3 Ab (BioLegend).

    Article Title: Inhibitory effect of IQ-1S, a selective c-Jun N-terminal kinase (JNK) inhibitor, on phenotypical and cytokine-producing characteristics in human macrophages and T-cells.
    Article Snippet: .. Cell cultures 116 Isolated CD14-positive cells were cultured at 1.0-1.5 × 106/ml in 24-well plates in the 117 presence or absence of bacterial LPS (1 μg/ml, Salmonella typhi, Pyrogenalum, Medgamal, 118 Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, 119 Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 120 50 μM of 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a 121 humidified CO2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0-1.5 × 10 6/ml in 122 the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against 123 human CD2, CD3, and CD28 (human T Cell Activation/Expansion Kit, MACS Miltenyi Biotec) 124 in the same cell culture medium for 48 h. Both monocytes/macrophages and T cells were 125 cultured in the absence (negative controls with DMSO) or presence (test cultures) of IQ-1S 126 dilutions specified above. ..

    Article Title: Directs effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on adaptive immunogenesis
    Article Snippet: Cells were counted using a Z2 Cell and Particle counter (Beckman Coulter Inc., Fullerton, USA). .. Cell cultures Isolated CD14-positive cells were cultured at 1.0–1.5 × 10 6 /ml in 24-well plates in the presence or absence of bacterial LPS (1.0 μg/ml, Salmonella typhi , Pyrogenalum, Medgamal, Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, Russian Academy of Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 5.0 × 10 −5 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a humidified CO 2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0–1.5 × 10 6 /ml in the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against human CD2, CD3, and CD28 (T Cell Activation/Expansion Kit, human, MACS Miltenyi Biotec) in the same cell culture medium for 48 h. Both Mc/Mph and T cell cultures were incubated in the presence (test cultures) or absence (negative controls) of a tenfold dilution series of recombinant human GM-CSF (0.01; 0.1; 1.0, and 10.0 ng/ml). .. Flow cytometry Surface characteristics of Mc/Mph cells were studied by staining with a cocktail of the following specific monoclonal antibody ( Ab )-based reagents: peridinin chlorophyll (PerCP)-labeled anti-CD14 (eBioscience, USA), fluorescein isothiocyanate (FITC)-labeled anti-CD16, phycoerythrin (PE)-labeled anti-CD119, allophycocyanin (APC)-labeled anti-CD124, and PE/Alexa Fluor® 488-labeled anti-CD197 (BioLegend, San Diego, CA).

    Cell Culture:

    Article Title: Inhibitory effect of IQ-1S, a selective c-Jun N-terminal kinase (JNK) inhibitor, on phenotypical and cytokine-producing characteristics in human macrophages and T-cells.
    Article Snippet: .. Cell cultures 116 Isolated CD14-positive cells were cultured at 1.0-1.5 × 106/ml in 24-well plates in the 117 presence or absence of bacterial LPS (1 μg/ml, Salmonella typhi, Pyrogenalum, Medgamal, 118 Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, 119 Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 120 50 μM of 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a 121 humidified CO2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0-1.5 × 10 6/ml in 122 the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against 123 human CD2, CD3, and CD28 (human T Cell Activation/Expansion Kit, MACS Miltenyi Biotec) 124 in the same cell culture medium for 48 h. Both monocytes/macrophages and T cells were 125 cultured in the absence (negative controls with DMSO) or presence (test cultures) of IQ-1S 126 dilutions specified above. ..

    Article Title: Directs effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on adaptive immunogenesis
    Article Snippet: Cells were counted using a Z2 Cell and Particle counter (Beckman Coulter Inc., Fullerton, USA). .. Cell cultures Isolated CD14-positive cells were cultured at 1.0–1.5 × 10 6 /ml in 24-well plates in the presence or absence of bacterial LPS (1.0 μg/ml, Salmonella typhi , Pyrogenalum, Medgamal, Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, Russian Academy of Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 5.0 × 10 −5 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a humidified CO 2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0–1.5 × 10 6 /ml in the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against human CD2, CD3, and CD28 (T Cell Activation/Expansion Kit, human, MACS Miltenyi Biotec) in the same cell culture medium for 48 h. Both Mc/Mph and T cell cultures were incubated in the presence (test cultures) or absence (negative controls) of a tenfold dilution series of recombinant human GM-CSF (0.01; 0.1; 1.0, and 10.0 ng/ml). .. Flow cytometry Surface characteristics of Mc/Mph cells were studied by staining with a cocktail of the following specific monoclonal antibody ( Ab )-based reagents: peridinin chlorophyll (PerCP)-labeled anti-CD14 (eBioscience, USA), fluorescein isothiocyanate (FITC)-labeled anti-CD16, phycoerythrin (PE)-labeled anti-CD119, allophycocyanin (APC)-labeled anti-CD124, and PE/Alexa Fluor® 488-labeled anti-CD197 (BioLegend, San Diego, CA).

    Activation Assay:

    Article Title: Inhibitory effect of IQ-1S, a selective c-Jun N-terminal kinase (JNK) inhibitor, on phenotypical and cytokine-producing characteristics in human macrophages and T-cells.
    Article Snippet: .. Cell cultures 116 Isolated CD14-positive cells were cultured at 1.0-1.5 × 106/ml in 24-well plates in the 117 presence or absence of bacterial LPS (1 μg/ml, Salmonella typhi, Pyrogenalum, Medgamal, 118 Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, 119 Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 120 50 μM of 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a 121 humidified CO2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0-1.5 × 10 6/ml in 122 the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against 123 human CD2, CD3, and CD28 (human T Cell Activation/Expansion Kit, MACS Miltenyi Biotec) 124 in the same cell culture medium for 48 h. Both monocytes/macrophages and T cells were 125 cultured in the absence (negative controls with DMSO) or presence (test cultures) of IQ-1S 126 dilutions specified above. ..

    Article Title: Directs effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on adaptive immunogenesis
    Article Snippet: Cells were counted using a Z2 Cell and Particle counter (Beckman Coulter Inc., Fullerton, USA). .. Cell cultures Isolated CD14-positive cells were cultured at 1.0–1.5 × 10 6 /ml in 24-well plates in the presence or absence of bacterial LPS (1.0 μg/ml, Salmonella typhi , Pyrogenalum, Medgamal, Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, Russian Academy of Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 5.0 × 10 −5 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a humidified CO 2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0–1.5 × 10 6 /ml in the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against human CD2, CD3, and CD28 (T Cell Activation/Expansion Kit, human, MACS Miltenyi Biotec) in the same cell culture medium for 48 h. Both Mc/Mph and T cell cultures were incubated in the presence (test cultures) or absence (negative controls) of a tenfold dilution series of recombinant human GM-CSF (0.01; 0.1; 1.0, and 10.0 ng/ml). .. Flow cytometry Surface characteristics of Mc/Mph cells were studied by staining with a cocktail of the following specific monoclonal antibody ( Ab )-based reagents: peridinin chlorophyll (PerCP)-labeled anti-CD14 (eBioscience, USA), fluorescein isothiocyanate (FITC)-labeled anti-CD16, phycoerythrin (PE)-labeled anti-CD119, allophycocyanin (APC)-labeled anti-CD124, and PE/Alexa Fluor® 488-labeled anti-CD197 (BioLegend, San Diego, CA).

    Article Title: Unstimulated Adult Human B Cells Include an IL-10+ Population with Suppressive Properties and an Activated Phenotype.
    Article Snippet: Cells were resuspended at a concentration of 1 × 106 cells/ml in OpTmizerTM CTSTM T cell expansion basal medium (Gibco, Grand Island, USA), stained with carboxyfluorescein succinimidyl ester (CFSE) and cultured in 96-well plates. .. Cells were stimulated with anti-biotin MACSiBead particles and biotinylated antibodies raised against human CD2, CD3, and CD28 (T cell activation/expansion kit, Miltenyi Biotec) at a bead-to-cell ratio of 1:2. ..

    Magnetic Cell Separation:

    Article Title: Inhibitory effect of IQ-1S, a selective c-Jun N-terminal kinase (JNK) inhibitor, on phenotypical and cytokine-producing characteristics in human macrophages and T-cells.
    Article Snippet: .. Cell cultures 116 Isolated CD14-positive cells were cultured at 1.0-1.5 × 106/ml in 24-well plates in the 117 presence or absence of bacterial LPS (1 μg/ml, Salmonella typhi, Pyrogenalum, Medgamal, 118 Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, 119 Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 120 50 μM of 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a 121 humidified CO2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0-1.5 × 10 6/ml in 122 the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against 123 human CD2, CD3, and CD28 (human T Cell Activation/Expansion Kit, MACS Miltenyi Biotec) 124 in the same cell culture medium for 48 h. Both monocytes/macrophages and T cells were 125 cultured in the absence (negative controls with DMSO) or presence (test cultures) of IQ-1S 126 dilutions specified above. ..

    Article Title: Directs effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on adaptive immunogenesis
    Article Snippet: Cells were counted using a Z2 Cell and Particle counter (Beckman Coulter Inc., Fullerton, USA). .. Cell cultures Isolated CD14-positive cells were cultured at 1.0–1.5 × 10 6 /ml in 24-well plates in the presence or absence of bacterial LPS (1.0 μg/ml, Salmonella typhi , Pyrogenalum, Medgamal, Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, Russian Academy of Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 5.0 × 10 −5 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a humidified CO 2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0–1.5 × 10 6 /ml in the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against human CD2, CD3, and CD28 (T Cell Activation/Expansion Kit, human, MACS Miltenyi Biotec) in the same cell culture medium for 48 h. Both Mc/Mph and T cell cultures were incubated in the presence (test cultures) or absence (negative controls) of a tenfold dilution series of recombinant human GM-CSF (0.01; 0.1; 1.0, and 10.0 ng/ml). .. Flow cytometry Surface characteristics of Mc/Mph cells were studied by staining with a cocktail of the following specific monoclonal antibody ( Ab )-based reagents: peridinin chlorophyll (PerCP)-labeled anti-CD14 (eBioscience, USA), fluorescein isothiocyanate (FITC)-labeled anti-CD16, phycoerythrin (PE)-labeled anti-CD119, allophycocyanin (APC)-labeled anti-CD124, and PE/Alexa Fluor® 488-labeled anti-CD197 (BioLegend, San Diego, CA).

    Recombinant:

    Article Title: Directs effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on adaptive immunogenesis
    Article Snippet: Cells were counted using a Z2 Cell and Particle counter (Beckman Coulter Inc., Fullerton, USA). .. Cell cultures Isolated CD14-positive cells were cultured at 1.0–1.5 × 10 6 /ml in 24-well plates in the presence or absence of bacterial LPS (1.0 μg/ml, Salmonella typhi , Pyrogenalum, Medgamal, Scientific Research Institute of Epidemiology and Microbiology named after N. F. Gamalei, Russian Academy of Russia) in a serum-free cell culture medium TexMACSTM (Miltenyi Biotec) supplemented with 5.0 × 10 −5 2-mercaptoethanol (Acros Organics/Thermo Fisher Scientific, NJ, USA) in a humidified CO 2 incubator at 37oC for 24 h. Isolated T cells were cultured at 1.0–1.5 × 10 6 /ml in the presence or absence of MACSiBead particles conjugated with antibodies (Abs) against human CD2, CD3, and CD28 (T Cell Activation/Expansion Kit, human, MACS Miltenyi Biotec) in the same cell culture medium for 48 h. Both Mc/Mph and T cell cultures were incubated in the presence (test cultures) or absence (negative controls) of a tenfold dilution series of recombinant human GM-CSF (0.01; 0.1; 1.0, and 10.0 ng/ml). .. Flow cytometry Surface characteristics of Mc/Mph cells were studied by staining with a cocktail of the following specific monoclonal antibody ( Ab )-based reagents: peridinin chlorophyll (PerCP)-labeled anti-CD14 (eBioscience, USA), fluorescein isothiocyanate (FITC)-labeled anti-CD16, phycoerythrin (PE)-labeled anti-CD119, allophycocyanin (APC)-labeled anti-CD124, and PE/Alexa Fluor® 488-labeled anti-CD197 (BioLegend, San Diego, CA).



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